Coding

Part:BBa_K5301011:Design

Designed by: Zihan Wen   Group: iGEM24_BNU-China   (2024-09-22)


spMSP1D1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 496
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 496
    Illegal NheI site found at 64
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 496
    Illegal BglII site found at 924
    Illegal BamHI site found at 97
    Illegal XhoI site found at 820
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 496
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 496
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Realize the self circularization of MSP and reference the ApoA-1 sequence to enable efficient binding with phospholipids.


Source

It's genetically engineered based on the Apolipoprotein A-1(ApoA-1).

References